| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Strikers - 01 Strikers CN 09 |
0437-01CB16-0259 | Cody Naylor |
| A2 | La Roca South La Roca '09B-A Ferreira |
0436-03PB16-1712 | Alcides Ferreira |
| A3 | Cottonwood FC - 01 Cottonwood FC B09 Red ZL |
0131-01BB16-0023 | Zachary Lancaster |
| A4 | Impact United Competitive Impact IC 09B |
0101-01CB16-7298 | Isak Ceribasic |
| A5 | Metasport FC MetaSport FC 09B JH (SFC) |
0430-05CB16-0005 | Jordan Hoffman |
| A6 | Avalanche SL/DW Avalanche Boys 09 EA |
0446-02C3B16-0062 | Ethan Liefting |
| A7 | Blast Soccer Club - 01 Blast 09 Blue |
0140-011B16-0019 | Shawn Kennedy |
| A8 | Avalanche SL/DW Avalanche Boys 09 Black DW |
0446-02CB16-0202 | Chris Keddington |